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micro bio spintm p 30 gel column  (Bio-Rad)


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    Structured Review

    Bio-Rad micro bio spintm p 30 gel column
    Micro Bio Spintm P 30 Gel Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 364 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/micro+bio+spintm+p+30+gel+columns/Micro+Bio-Spin+P-30+Gel+Columns/pm41547900-317-13-18
    Average 96 stars, based on 364 article reviews
    micro bio spintm p 30 gel column - by Bioz Stars, 2026-10
    96/100 stars

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    other:

    Article Title: CREPT is required for the metastasis of triple-negative breast cancer through a co-operational-chromatin loop-based gene regulation
    Article Snippet: Micro Bio-SpinTM P-30 Gel Columns , 732–6250 , Bio-Rad.

    Article Title: A CDK11-dependent RNA polymerase II pause-checkpoint precedes CDK9-mediated transition to transcriptional elongation
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER μMACS Streptavidin Kit Miltenyi Biotec Cat#130-074-101 NucleoSpin Gel and PCR Clean-up Mini kit Macherey-Nagel Cat#740609 NucleoSpin Plasmid Mini kit Macherey-Nagel Cat#740588 Deposited data RNA-seq data This paper GSE280102 ChIP-seq data This paper GSE280208 PRO- and TT-seq data This paper GSE280103 Mass spectrometry data This paper PXD066230 Experimental models: Cell lines Human: THP-1 American Type Cell Culture® (ATCC®) Cat#TIB-202; RRID: CVCL_0006 Human: MV4;11 ATCC® Cat#CRL-9591; RRID: CVCL_0064 Human: BJ-T ATCC® Cat#CRL-2522; RRID: CVCL_6573 Human: MM1.S ATCC® Cat#CRL-2974; RRID: CVCL_8792 Human: OCI-AML3 German Collection of Microorganisms and Cell Cultures GmbH (DSMZ) Cat#ACC 582; RRID: CVCL_1844 Human: HEK293T ATCC® Cat#CRL-11268; RRID: CVCL_0063 Human: HCT116 ATCC® Cat#CCL-247; RRID: CVCL_0291 Drosophila melanogaster: S2 ATCC® Cat#CRL-1963; RRID: CVCL_Z232 Murine: Mll-Af9 Nras G12D mCherry Baker et al., 30 ; Peter MacCallum Cancer Centre N/A Murine: Eμ-Myc 4242/3.2 Shortt et al., 103 ; Peter MacCallum Cancer Centre N/A Murine: Eμ-Myc 6066/2.1 Shortt et al., 103 ;Peter MacCallum Cancer Centre N/A Murine: Eμ-Myc 299/2.1 Shortt et al., 103 ; Peter MacCallum Cancer Centre N/A Spodoptera frugiperda: Sf21 Professor Mark Hogarth, Burnet Institute, Melbourne VIC 3000, Australia RRID: CVCL_0518 Experimental models: Organisms/strains Mice: C57Bl/6 The Walter and Eliza Hall Institute, Melbourne VIC 3000, Australia N/A Oligonucleotides Scrambled (SCR) sgRNA for Human cells GCCTAGTCTCGGTAAGAGTG Doench et al. 104 Non-Targeting Control sgRNA targeting Human CDK11A/B (for CDK11A/B knockout)sense: TCCCAC ATCACCGAACGATGAGAGantisense: AAACCTCTCATCGTTCGGTGATGT Doench et al. 104 ID_2782 sgRNA targeting Human CDK11B (for CDK11B G579S homology-directed-repair) GGGTGACTTCGGGCTGGCGC This paper N/A DNA donor template for Human CDK11B G579S mutationAGCCACGCCGGCATCCTCAAG GTGAGCCCCCCTCCGAGTGGCCCATC CCAGGGAGACCTGCCGGGGCCCACTC ACAGCCGCCCATCTGTCGTTGCAGGTG AGTGATTTTGGACTGGCGCGGGAGTAC GGATCCCCTCTGAAGGCCTACACCCCG GTCGTGGTGACCCTGTGGTACCGCGCC CCAGAGCTGCTGCTTGGTG This paper N/A (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 85, 1–20.e1–e13, September 4, 2025 e3

    Article Title: A CDK11-dependent RNA polymerase II pause-checkpoint precedes CDK9-mediated transition to transcriptional elongation
    Article Snippet: To enrich biotin-CTP-incorporating RNA, hydrolysed RNA was incubated with 30μL streptavidin DynaBeads (Invitrogen 11206D; prewashed with 1) 100mM NaOH, 50mM NaCl and 2) 100mM NaCl and resuspended in binding buffer (100mM Tris-HCl pH 7.4, 300mM NaCl, 0.1% (v/v) TritonX-100) at room temperature for 20 minutes (tumbling at 8rpm).

    Generated:

    Article Title: Altered socio-affective communication and amygdala development in mice with protocadherin10-deficient interneurons.
    Article Snippet: In vitro transcription was performed by adding linearized plasmid, Sp6 polymerase (Roche), DIG labelling mix (Roche) and RNase inhibitor (Roche) in 1× reaction buffer (Roche) and incubated overnight at 37°C. .. Generated probes were purified using Micro Bio-SpinTM P-30 Gel Columns with RNase-free Tris Buffer (BioRad) according to the manufacturer’s protocol and stored at −80°C. .. In situ hybridization (ISH) was performed using an automated system (VENTANA Discovery, Roche).

    Purification:

    Article Title: Altered socio-affective communication and amygdala development in mice with protocadherin10-deficient interneurons.
    Article Snippet: In vitro transcription was performed by adding linearized plasmid, Sp6 polymerase (Roche), DIG labelling mix (Roche) and RNase inhibitor (Roche) in 1× reaction buffer (Roche) and incubated overnight at 37°C. .. Generated probes were purified using Micro Bio-SpinTM P-30 Gel Columns with RNase-free Tris Buffer (BioRad) according to the manufacturer’s protocol and stored at −80°C. .. In situ hybridization (ISH) was performed using an automated system (VENTANA Discovery, Roche).

    Article Title: Regulation of the Yolk Microtubule and Actin Cytoskeleton by Dachsous Cadherins during Zebrafish Epiboly
    Article Snippet: The process involved in assembling RVD-containing repeats and subcloning TALE repeats into modified TALE nuclease expression vectors was performed using the REAL Assembly TALEN Kit (Addgene TALEN kit 1000000017) (Sander et al., 2011). .. TALEN RNAs were synthesized using SP6 mMessage mMachine Kit (Ambion) and purified by Micro Bio-SpinTM P-30 Gel columns (Bio-Rad). .. To create a dchs1b targeting construct that is capable to induce an insertion of a long DNA fragment into the dchs1b stop codon site by TALEN-mediated homologous recombination, we first amplified a genomic DNA fragment (3,908 bp) containing the dchs1b stop codon by PCR and subcloned it into pSMART®HCKan vector (Lucigne).

    Synthesized:

    Article Title: Regulation of the Yolk Microtubule and Actin Cytoskeleton by Dachsous Cadherins during Zebrafish Epiboly
    Article Snippet: The process involved in assembling RVD-containing repeats and subcloning TALE repeats into modified TALE nuclease expression vectors was performed using the REAL Assembly TALEN Kit (Addgene TALEN kit 1000000017) (Sander et al., 2011). .. TALEN RNAs were synthesized using SP6 mMessage mMachine Kit (Ambion) and purified by Micro Bio-SpinTM P-30 Gel columns (Bio-Rad). .. To create a dchs1b targeting construct that is capable to induce an insertion of a long DNA fragment into the dchs1b stop codon site by TALEN-mediated homologous recombination, we first amplified a genomic DNA fragment (3,908 bp) containing the dchs1b stop codon by PCR and subcloned it into pSMART®HCKan vector (Lucigne).



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